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A separate multilevel mixed-effects linear regression was utilized to investigate potential differences between the baseline measure and 24 h after baseline in each condition
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The eluate was dialysed at 4 °C overnight in dialysis buffer (50 mM Tris pH 8.0, 100 mM NaCl, 5% (v/v) glycerol, 15 mM imidazole and 4 mM BME) using a 10-kDa molecular weight cutoff dialysis cassette (Thermo Scientific, 69570)
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