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For single-stranded DNA digestion, Acr proteins (0.4, 0.8, 1.6, or 3.2 μM final concentration) were pre-incubated with 0.64 μM Cas2/3 in reaction buffer (20 mM HEPES, pH 7.5, 100 mM KCl, 5% glycerol, 1 mM TCEP) at 37 °C for 30 min

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Hyper methylation in LC persisted for one year after hospitalization [66]