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The definition finalized in that final rule states that the term “BFSFs” means fees paid by a manufacturer to an entity, that represent fair market value for a bona fide, itemized service actually performed on behalf of the manufacturer that the manufacturer would otherwise perform (or contract for) in the absence of the service arrangement, and that are not passed on in whole or in part to a client or customer of an entity, whether or not the entity takes title to the drug

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found that human liver NNMT had Km values of 430 and 1.8 μM for NAM and SAM respectively by using the radiochemical enzymatic assay at 37 °C, which is based on the conversion of NAM to radioactively labeled 1-MNAM with [methyl- 14 C]-SAM as the methyl donor [5]